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Showing Results For: Molekularbiologie Reagenzien (Serva)

Molekularbiologie Reagenzien (Serva)



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39751.01, Omnilab

39751.01, Omnilab

7.5 M stock solution (ammonium acetate, cat. no. 39750: 578.1 g/l) for precipitation of DNA. DNase/RNase not detected.Short oligonucleotides and free dNTPs do not coprecipitate with DNA when precipitated with NH4OAc. Two consecutive precipitation steps are enough to remove 99 % of free dNTPs. 7.5 M stock solution (ammonium acetate, cat. no. 39750: 578.1 g/l) for precipitation of DNA. DNase/RNase not detected.Short oligonucleotides and free dNTPs do not coprecipitate with DNA when precipitated with NH4OAc. Two consecutive precipitation steps are enough to remove 99 % of free dNTPs.

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New
39803.02, Omnilab

39803.02, Omnilab

SERVA DNA Stain G is a safer alternative to traditional ethidium bromide stain for detecting nucleic acids in agarose gels. It is at least as sensitive as ethidium bromide and can be used in exactly the same way in agarose gel electrophoresis. SERVA DNA Stain G can be added to the gel solution, working dilution is 1:20,000 to 1:50,000.SERVA DNA Stain G emits green fluorescence when bound to DNA or RNA. It has one fluorescence excitation maximum at ca. 300 nm and another at ca. 450 nm when bound to nucleic acid.SERVA DNA Stain G is non-carcinogenic and according to the AMES test it causes significantly fewer mutations than ethidium bromide. SERVA DNA Stain G is a safer alternative to traditional ethidium bromide stain for detecting nucleic acids in agarose gels. It is at least as sensitive as ethidium bromide and can be used in exactly the same way in agarose gel electrophoresis. SERVA DNA Stain G can be added to the gel solution, working dilution is 1:20,000 to 1:50,000.SERVA DNA Stain G emits green fluorescence when bound to DNA or RNA. It has one fluorescence excitation maximum at ca. 300 nm and another at ca. 450 nm when bound to nucleic acid.SERVA DNA Stain G is non-carcinogenic and according to the AMES test it causes significantly fewer mutations than ethidium bromide.

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New
39804.01, Omnilab

39804.01, Omnilab

SERVA DNA Stain Clear G is a new, non-carcinogenic, much more sensitive and convenient version of our SERVA DNA Stain G. It can be used instead of highly carcinogenic ethidium bromide for detecting nucleic acids in agarose gels.SERVA DNA Stain Clear G emits green fluorescence when bound to DNA or RNA. It has two secondary fluorescence excitation peaks (ca. 270 nm and 295 nm) and one strong excitation peak centered around 490 nm. The fluorescence emission is similar to EtBr at ca. 530 nm when bound to nucleic acid.Pre-casting and post-staining protocols are applicable.1 ml of this stain is sufficient for 17 - 25 L of agarose gel. SERVA DNA Stain Clear G is a new, non-carcinogenic, much more sensitive and convenient version of our SERVA DNA Stain G. It can be used instead of highly carcinogenic ethidium bromide for detecting nucleic acids in agarose gels.SERVA DNA Stain Clear G emits green fluorescence when bound to DNA or RNA. It has two secondary fluorescence excitation peaks (ca. 270 nm and 295 nm) and one strong excitation peak centered around 490 nm. The fluorescence emission is similar to EtBr at ca. 530 nm when bound to nucleic acid.Pre-casting and post-staining protocols are applicable.1 ml of this stain is sufficient for 17 - 25 L of agarose gel.

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New
39766.01, Omnilab

39766.01, Omnilab

Suitable as a carrier molecule for DNA and RNA, replacing tRNA and sonicated DNA.DNase/RNase not detected. Suitable as a carrier molecule for DNA and RNA, replacing tRNA and sonicated DNA.DNase/RNase not detected.

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New
39757.02, Omnilab

39757.02, Omnilab

Dimethyl sulfoxide (DMSO) is a highly active solvent and pharmaceutical vehicle. In cell culture DMSO is used for freezing cells. The solvent is as well applied in gradient centrifugation (1) and determination of cysteine and cystine in proteins (2).DNase/RNase not detected. Dimethyl sulfoxide (DMSO) is a highly active solvent and pharmaceutical vehicle. In cell culture DMSO is used for freezing cells. The solvent is as well applied in gradient centrifugation (1) and determination of cysteine and cystine in proteins (2).DNase/RNase not detected.

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New
39759.03, Omnilab

39759.03, Omnilab

Dithiothreitol, as well called DTT or Cleland's reagent, is a widely used reagent for maintaining thiol (SH) groups in reduced state. DTT quantitatively reduces disulfide groups (1) and is effective in sample buffers for reducing protein disulfide bonds prior to SDS gel electrophoresis (SDS-PAGE).It is not only less pungent and less toxic than 2-mercaptoethanol, but as well a seven fold lower concentration of DTT (100 mM) than of 2-mercaptoethanol (5 % v/v, 700 mM) is needed.DNase/RNase not detected. Oxidized DTT max. 0.5 %. Hygroscopic. Dithiothreitol, as well called DTT or Cleland's reagent, is a widely used reagent for maintaining thiol (SH) groups in reduced state. DTT quantitatively reduces disulfide groups (1) and is effective in sample buffers for reducing protein disulfide bonds prior to SDS gel electrophoresis (SDS-PAGE).It is not only less pungent and less toxic than 2-mercaptoethanol, but as well a seven fold lower concentration of DTT (100 mM) than of 2-mercaptoethanol (5 % v/v, 700 mM) is needed.DNase/RNase not detected. Oxidized DTT max. 0.5 %. Hygroscopic.

Product Detail
New
39804.02, Omnilab

39804.02, Omnilab

SERVA DNA Stain Clear G is a new, non-carcinogenic, much more sensitive and convenient version of our SERVA DNA Stain G. It can be used instead of highly carcinogenic ethidium bromide for detecting nucleic acids in agarose gels.SERVA DNA Stain Clear G emits green fluorescence when bound to DNA or RNA. It has two secondary fluorescence excitation peaks (ca. 270 nm and 295 nm) and one strong excitation peak centered around 490 nm. The fluorescence emission is similar to EtBr at ca. 530 nm when bound to nucleic acid.Pre-casting and post-staining protocols are applicable.1 ml of this stain is sufficient for 17 - 25 L of agarose gel. SERVA DNA Stain Clear G is a new, non-carcinogenic, much more sensitive and convenient version of our SERVA DNA Stain G. It can be used instead of highly carcinogenic ethidium bromide for detecting nucleic acids in agarose gels.SERVA DNA Stain Clear G emits green fluorescence when bound to DNA or RNA. It has two secondary fluorescence excitation peaks (ca. 270 nm and 295 nm) and one strong excitation peak centered around 490 nm. The fluorescence emission is similar to EtBr at ca. 530 nm when bound to nucleic acid.Pre-casting and post-staining protocols are applicable.1 ml of this stain is sufficient for 17 - 25 L of agarose gel.

Product Detail
New
42553.01, Omnilab

42553.01, Omnilab

10 x concentrated aqueous solution.TAE Buffer is used for the electrophoresis of nucleic acids. TAE has a lower buffer capacity than TBE, however linear ds DNA tends to run faster in TAE than in TBE. 10 x concentrated aqueous solution.TAE Buffer is used for the electrophoresis of nucleic acids. TAE has a lower buffer capacity than TBE, however linear ds DNA tends to run faster in TAE than in TBE.

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New
39772.02, Omnilab

39772.02, Omnilab

Magnesium chloride is used as a source of magnesium ions in various molecular biology applications like PCR reactions and for the preparation of competent cells for transformation. It is an essential co-factor in many enzymes, including DNase, some restriction enzymes, and Ribonuclease H.DNase/RNase not detected. Magnesium chloride is used as a source of magnesium ions in various molecular biology applications like PCR reactions and for the preparation of competent cells for transformation. It is an essential co-factor in many enzymes, including DNase, some restriction enzymes, and Ribonuclease H.DNase/RNase not detected.

Product Detail
New
39759.02, Omnilab

39759.02, Omnilab

Dithiothreitol, as well called DTT or Cleland's reagent, is a widely used reagent for maintaining thiol (SH) groups in reduced state. DTT quantitatively reduces disulfide groups (1) and is effective in sample buffers for reducing protein disulfide bonds prior to SDS gel electrophoresis (SDS-PAGE).It is not only less pungent and less toxic than 2-mercaptoethanol, but as well a seven fold lower concentration of DTT (100 mM) than of 2-mercaptoethanol (5 % v/v, 700 mM) is needed.DNase/RNase not detected. Oxidized DTT max. 0.5 %. Hygroscopic. Dithiothreitol, as well called DTT or Cleland's reagent, is a widely used reagent for maintaining thiol (SH) groups in reduced state. DTT quantitatively reduces disulfide groups (1) and is effective in sample buffers for reducing protein disulfide bonds prior to SDS gel electrophoresis (SDS-PAGE).It is not only less pungent and less toxic than 2-mercaptoethanol, but as well a seven fold lower concentration of DTT (100 mM) than of 2-mercaptoethanol (5 % v/v, 700 mM) is needed.DNase/RNase not detected. Oxidized DTT max. 0.5 %. Hygroscopic.

Product Detail
New
39766.02, Omnilab

39766.02, Omnilab

Suitable as a carrier molecule for DNA and RNA, replacing tRNA and sonicated DNA.DNase/RNase not detected. Suitable as a carrier molecule for DNA and RNA, replacing tRNA and sonicated DNA.DNase/RNase not detected.

Product Detail
New
42555.01, Omnilab

42555.01, Omnilab

20x SSC Buffer is a versatile buffer for transfer, blocking and hybridisation in Southern and Northern blotting, for in situ hybridisations and for neutral transfer of RNA. The concentrated powder can be easily dissolved in sterile water. The 20x SSC concentrate can be used pure or in a dilution with concentrations up to 0.5x.DNase/RNase not detectable. 20x SSC Buffer is a versatile buffer for transfer, blocking and hybridisation in Southern and Northern blotting, for in situ hybridisations and for neutral transfer of RNA. The concentrated powder can be easily dissolved in sterile water. The 20x SSC concentrate can be used pure or in a dilution with concentrations up to 0.5x.DNase/RNase not detectable.

Product Detail
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