Deliver to
United Kingdom
Mixture of 7 different inhibitors dissolved in water, suitable for the inhibition of acid and alkaline phosphatases, protein phosphatases 2A, 2B and 2C, phosphoprotein phosphatase, protein-tyrosine phosphatase, and serine/threonine phosphatase. Contains EDTA, ß-glycerophosphate-disodium salt, imidazole, sodium fluoride, sodium molybdate, sodium-ortho-vanadate, and sodium tartrate. 1 ml solution is suitable for the treatment of 100 ml tissue extract. Mixture of 7 different inhibitors dissolved in water, suitable for the inhibition of acid and alkaline phosphatases, protein phosphatases 2A, 2B and 2C, phosphoprotein phosphatase, protein-tyrosine phosphatase, and serine/threonine phosphatase. Contains EDTA, ß-glycerophosphate-disodium salt, imidazole, sodium fluoride, sodium molybdate, sodium-ortho-vanadate, and sodium tartrate. 1 ml solution is suitable for the treatment of 100 ml tissue extract.
Product Detail
Protease Inhibitor Mix HP Plus is an effective protection against proteolytic degradation during affinity purification of recombinant polyHis-tag proteins and for other applications, where metal-chelators should be avoided. The mixture of six protease inhibitors has a broad range of activity against aspartate-, cysteine-, and serine proteases as well as aminopeptidases, Thermolysin and other microbial metalloendoproteases. Protease Inhibitor Mix HP Plus contains AEBSF, Bestatin, E-64, Leupeptin, Pepstatin A, and Phosphoramidon.Supplied as a kit, each vial is provided with an extra vial of 1 ml DMSO.The content of 1 vial dissolved in 1 ml DMSO will give a 100-fold concentrate, sufficient for 99 ml extract. Protease Inhibitor Mix HP Plus is an effective protection against proteolytic degradation during affinity purification of recombinant polyHis-tag proteins and for other applications, where metal-chelators should be avoided. The mixture of six protease inhibitors has a broad range of activity against aspartate-, cysteine-, and serine proteases as well as aminopeptidases, Thermolysin and other microbial metalloendoproteases. Protease Inhibitor Mix HP Plus contains AEBSF, Bestatin, E-64, Leupeptin, Pepstatin A, and Phosphoramidon.Supplied as a kit, each vial is provided with an extra vial of 1 ml DMSO.The content of 1 vial dissolved in 1 ml DMSO will give a 100-fold concentrate, sufficient for 99 ml extract.
Product Detail
Protease Inhibitor Mix M is an effective protection against proteolytic degradation in the preparation of extracts from mammalian tissue. The mixture of six protease inhibitors has a broad range of activity against aspartate-, cysteine-, serine- and metallo proteases as well as aminopeptidases. Protease Inhibitor Mix M is free of EDTA and contains AEBSF, Aprotinin, Bestatin, E-64, Leupeptin and Pepstatin A.Supplied as a kit: each vial is provided with an extra vial of 1 ml DMSO.The content of 1 vial dissolved in 1 ml DMSO will give a 100-fold concentrate, sufficient for 99 ml extract. Protease Inhibitor Mix M is an effective protection against proteolytic degradation in the preparation of extracts from mammalian tissue. The mixture of six protease inhibitors has a broad range of activity against aspartate-, cysteine-, serine- and metallo proteases as well as aminopeptidases. Protease Inhibitor Mix M is free of EDTA and contains AEBSF, Aprotinin, Bestatin, E-64, Leupeptin and Pepstatin A.Supplied as a kit: each vial is provided with an extra vial of 1 ml DMSO.The content of 1 vial dissolved in 1 ml DMSO will give a 100-fold concentrate, sufficient for 99 ml extract.
Product Detail
Mixture of 5 water-soluble inhibitors against acid and alkaline phosphatases, protein phosphatases 2A, 2B and 2C, phosphoprotein phosphatase, and protein-tyrosine phosphatase.Contains imidazole, sodium fluoride, sodium molybdate, sodium-ortho-vanadate, and sodium tartrate. The content of 1 vial dissolved in 1 ml water will give a 100-fold concentrate suitable for the treatment of 100 ml tissue extract. Mixture of 5 water-soluble inhibitors against acid and alkaline phosphatases, protein phosphatases 2A, 2B and 2C, phosphoprotein phosphatase, and protein-tyrosine phosphatase.Contains imidazole, sodium fluoride, sodium molybdate, sodium-ortho-vanadate, and sodium tartrate. The content of 1 vial dissolved in 1 ml water will give a 100-fold concentrate suitable for the treatment of 100 ml tissue extract.
Product Detail
Protease Inhibitor Mix HP Plus is an effective protection against proteolytic degradation during affinity purification of recombinant polyHis-tag proteins and for other applications, where metal-chelators should be avoided. The mixture of six protease inhibitors has a broad range of activity against aspartate-, cysteine-, and serine proteases as well as aminopeptidases, Thermolysin and other microbial metalloendoproteases. Protease Inhibitor Mix HP Plus contains AEBSF, Bestatin, E-64, Leupeptin, Pepstatin A, and Phosphoramidon.Supplied as a kit, each vial is provided with an extra vial of 1 ml DMSO.The content of 1 vial dissolved in 1 ml DMSO will give a 100-fold concentrate, sufficient for 99 ml extract. Protease Inhibitor Mix HP Plus is an effective protection against proteolytic degradation during affinity purification of recombinant polyHis-tag proteins and for other applications, where metal-chelators should be avoided. The mixture of six protease inhibitors has a broad range of activity against aspartate-, cysteine-, and serine proteases as well as aminopeptidases, Thermolysin and other microbial metalloendoproteases. Protease Inhibitor Mix HP Plus contains AEBSF, Bestatin, E-64, Leupeptin, Pepstatin A, and Phosphoramidon.Supplied as a kit, each vial is provided with an extra vial of 1 ml DMSO.The content of 1 vial dissolved in 1 ml DMSO will give a 100-fold concentrate, sufficient for 99 ml extract.
Product Detail
Purified inhibitor of trypsin, chymotrypsin, plasmin and especially kallikrein. For in vitro inhibition of fibrinolytic activity in blood samples (1). Activity: min. 3 Ph. Eur. Units (PEU)/mg, based on dried substance. Purified inhibitor of trypsin, chymotrypsin, plasmin and especially kallikrein. For in vitro inhibition of fibrinolytic activity in blood samples (1). Activity: min. 3 Ph. Eur. Units (PEU)/mg, based on dried substance.
Product Detail
E-64 is an irreversible and highly selective cysteine (thiol) protease inhibitor during isolation and purification of proteins and enzymes. It inhibits calpain activation.The inhibition of thiol proteases by E-64 appears to be of a non-competitive nature between the SH components. The trans-epoxysuccinyl group (active moiety) of E-64 irreversibly binds to an active thiol group in many cysteine proteases, such as papain, actinidase, and cathepsins B, H, and L to form a thioether linkage. E-64 does not inhibit serine proteases (except trypsin) like other cysteine protease inhibitors. It does not react with the functional thiol group of non-protease enzymes. E-64 can be used as a ligand for affinity purification of cysteine proteases. Although binding of the inhibitor is no longer irreversible, the specificity is retained. Effects on metastasis formation in mice (5).Stock solution: 1 mM aqueous solutionWorking solution: 1 to10 µMAqueous stock solutions are stable for months at -20 °C. Diluted solutions are stable for days at neutral pH.E-64 is also soluble in DMSO, a 10 mM solution can be prepared in dry DMSO and stored at -20 °C. Dilutions can be made in culture medium or in 0.9 % sodium chloride for injection. E-64 is an irreversible and highly selective cysteine (thiol) protease inhibitor during isolation and purification of proteins and enzymes. It inhibits calpain activation.The inhibition of thiol proteases by E-64 appears to be of a non-competitive nature between the SH components. The trans-epoxysuccinyl group (active moiety) of E-64 irreversibly binds to an active thiol group in many cysteine proteases, such as papain, actinidase, and cathepsins B, H, and L to form a thioether linkage. E-64 does not inhibit serine proteases (except trypsin) like other cysteine protease inhibitors. It does not react with the functional thiol group of non-protease enzymes. E-64 can be used as a ligand for affinity purification of cysteine proteases. Although binding of the inhibitor is no longer irreversible, the specificity is retained. Effects on metastasis formation in mice (5).Stock solution: 1 mM aqueous solutionWorking solution: 1 to10 µMAqueous stock solutions are stable for months at -20 °C. Diluted solutions are stable for days at neutral pH.E-64 is also soluble in DMSO, a 10 mM solution can be prepared in dry DMSO and stored at -20 °C. Dilutions can be made in culture medium or in 0.9 % sodium chloride for injection.
Product Detail
Purified inhibitor of trypsin, chymotrypsin, plasmin and especially kallikrein. For in vitro inhibition of fibrinolytic activity in blood samples (1). Activity: min. 3 Ph. Eur. Units (PEU)/mg, based on dried substance. Purified inhibitor of trypsin, chymotrypsin, plasmin and especially kallikrein. For in vitro inhibition of fibrinolytic activity in blood samples (1). Activity: min. 3 Ph. Eur. Units (PEU)/mg, based on dried substance.
Product Detail
Protease Inhibitor Mix HP is an effective protection against proteolytic degradation during affinity purification of recombinant polyHis-tag proteins. It can also be used wherever complexing agents are to be avoided.Protease Inhibitor Mix HP inhibits cysteine and serine proteases. It contains the four water-soluble protease inhibitors AEBSF, aprotinin, E-64, and leupeptin and is free of metal chelating complexing agents.The content of one tube dissolved in 1 ml distilled water gives a 100-fold concentrate, which is sufficient for 99 ml extract. Protease Inhibitor Mix HP is an effective protection against proteolytic degradation during affinity purification of recombinant polyHis-tag proteins. It can also be used wherever complexing agents are to be avoided.Protease Inhibitor Mix HP inhibits cysteine and serine proteases. It contains the four water-soluble protease inhibitors AEBSF, aprotinin, E-64, and leupeptin and is free of metal chelating complexing agents.The content of one tube dissolved in 1 ml distilled water gives a 100-fold concentrate, which is sufficient for 99 ml extract.
Product Detail
Mixture of 7 different inhibitors dissolved in water, suitable for the inhibition of acid and alkaline phosphatases, protein phosphatases 2A, 2B and 2C, phosphoprotein phosphatase, protein-tyrosine phosphatase, and serine/threonine phosphatase. Contains EDTA, ß-glycerophosphate-disodium salt, imidazole, sodium fluoride, sodium molybdate, sodium-ortho-vanadate, and sodium tartrate. 1 ml solution is suitable for the treatment of 100 ml tissue extract. Mixture of 7 different inhibitors dissolved in water, suitable for the inhibition of acid and alkaline phosphatases, protein phosphatases 2A, 2B and 2C, phosphoprotein phosphatase, protein-tyrosine phosphatase, and serine/threonine phosphatase. Contains EDTA, ß-glycerophosphate-disodium salt, imidazole, sodium fluoride, sodium molybdate, sodium-ortho-vanadate, and sodium tartrate. 1 ml solution is suitable for the treatment of 100 ml tissue extract.
Product Detail
Inhibits trypsin and chymotrypsin (1). Non-inhibitory to cholineesterase. Less toxic than diisopropylfluorophosphate (2). Inactivation of PMSF in buffer (3). Inhibits trypsin and chymotrypsin (1). Non-inhibitory to cholineesterase. Less toxic than diisopropylfluorophosphate (2). Inactivation of PMSF in buffer (3).
Product Detail
Mixture of 5 water-soluble inhibitors against acid and alkaline phosphatases, protein phosphatases 2A, 2B and 2C, phosphoprotein phosphatase, and protein-tyrosine phosphatase.Contains imidazole, sodium fluoride, sodium molybdate, sodium-ortho-vanadate, and sodium tartrate. The content of 1 vial dissolved in 1 ml water will give a 100-fold concentrate suitable for the treatment of 100 ml tissue extract. Mixture of 5 water-soluble inhibitors against acid and alkaline phosphatases, protein phosphatases 2A, 2B and 2C, phosphoprotein phosphatase, and protein-tyrosine phosphatase.Contains imidazole, sodium fluoride, sodium molybdate, sodium-ortho-vanadate, and sodium tartrate. The content of 1 vial dissolved in 1 ml water will give a 100-fold concentrate suitable for the treatment of 100 ml tissue extract.
Product Detail© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
