Non-toxic and non-carcinogenic dyes for fluorescent staining of nucleic acids in agarose gels and polyacrylamide gels. Highly sensitive and applied just like ethidium bromide. The ROTI ® GelStain dyes are used as additives to the gel solution and the running buffer, partly also post-run staining is possible. Both ROTI ® GelStain dyes can be excited by UV light and by blue light. Resulting signals may be detected by ethidium bromide broadband photo filters. ROTI ® GelStain 20 000x conc., ready-to-use ROTI ® GelStain is a versatile staining reagent for detection of all nucleic acids in agarose and polyacrylamide gels. Green fluorescence Alternative for ethidium bromide, non-toxic and non-mutagenic For dsDNA, ssDNA and RNA Sensitivity comparable to ethidium bromide (approx. 0.3 ng / band) For use with common broad-band ethidium bromide photo filters Excitation via UV light (302 nm) and blue light (490 nm) Compatible with all usual down-stream applications The dye can be used in a similar manner as ethidium bromide. By adding the dye to the liquid gel, the nucleic acids are stained during the gel run. ROTI ® GelStain has a sensitivity of 0.2 ng/band and is thus even slightly more sensitive than ethidium bromide. When bound to nucleic acids in agarose gels, ROTI ® GelStain emits a brightly green fluorescence that may be documented by all usual broadband ethidium bromide or SYBR ® Green foto filters. ROTI ® -GelStain is compatible with all standard down-stream applications and has been successfully tested for use with gel extraction, ligation, transformation and transfection. Wavelength Excitation maximum (bound to DNA): 302 nm (UV) and 490 nm (blue light) Emission maximum (bound to DNA): 520 nm Figure: Amplicons of a mouse genotyping (left), and restriction digested DNA (right), respectively, separated in 2,5 and 1 % agarose ROTI ® garose NEEO with ROTI ® GelStain (5 µl / 100 ml agarose). Pictures were taken using standard ethidium bromide photo filters. With kind permission of Prof. R. Slany, Dept. of Genetics, Friedrich Alexander University, Erlangen.
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