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5-Bromo-4-chloro-3-indolyl phosphate disodium salt ≥99 % In combination with NBT ideal for colorimetric detection of alkaline phosphatase activity, in particular in immunoblot procedures.
Product Detail
p -Nitrotetrazolium blue chloride ≥98 %, p.a. Used in combination with BCIP-toluidine salt (X-phosphate) for detection of alkaline phosphatase activity by producing an indigo dye precipitate. Colour enhancer for staining with alkalic phosphatase. Mechanism During dimerisation of ketonized X-Phos, H + ions are set free which reduce NBT to purple-coloured diformazan. Both dyes precipitate in the direct vicinity of the phosphatase, hence staining this site in a dark violet. Store at +4 °C. Please also see assay protocol for use of BCIP/NBT in immunoblot procedures.
Product Detail
6-Chloro-3-indoxyl phosphate p -toluidine salt ≥98 %, for biochemistry
Product Detail
5-Bromo-4-chloro-3-indolyl phosphate disodium salt ≥99 % In combination with NBT ideal for colorimetric detection of alkaline phosphatase activity, in particular in immunoblot procedures.
Product Detail
6-Chloro-3-indoxyl phosphate p -toluidine salt ≥98 %, for biochemistry
Product Detail
p -Nitrotetrazolium blue chloride ≥98 %, p.a. Used in combination with BCIP-toluidine salt (X-phosphate) for detection of alkaline phosphatase activity by producing an indigo dye precipitate. Colour enhancer for staining with alkalic phosphatase. Mechanism During dimerisation of ketonized X-Phos, H + ions are set free which reduce NBT to purple-coloured diformazan. Both dyes precipitate in the direct vicinity of the phosphatase, hence staining this site in a dark violet. Store at +4 °C. Please also see assay protocol for use of BCIP/NBT in immunoblot procedures.
Product Detail
5-Bromo-4-chloro-3-indolyl phosphate disodium salt ≥99 % In combination with NBT ideal for colorimetric detection of alkaline phosphatase activity, in particular in immunoblot procedures.
Product Detail
p -Nitrotetrazolium blue chloride ≥98 %, p.a. Used in combination with BCIP-toluidine salt (X-phosphate) for detection of alkaline phosphatase activity by producing an indigo dye precipitate. Colour enhancer for staining with alkalic phosphatase. Mechanism During dimerisation of ketonized X-Phos, H + ions are set free which reduce NBT to purple-coloured diformazan. Both dyes precipitate in the direct vicinity of the phosphatase, hence staining this site in a dark violet. Store at +4 °C. Please also see assay protocol for use of BCIP/NBT in immunoblot procedures.
Product Detail
5-Bromo-4-chloro-3-indolyl phosphate p -toluidine salt ≥99 %, BioScience Grade In combination with NBT ideal for colorimetric detection of alkaline phosphatase activity, in immunohistochemical procedures. Mechanism By enzymatic dephosphorylation BCIP is converted to indoxyl which subsequently tautomerizes to the ketone. In basic pH values, this ketone dimerizes and, in doing so, releases H + ions. The dye precipitates in the direct vicinity of the phosphatase, hence staining this site in a weak blue colour. Enhencement and darkening of this colour is achieved by adding NBT (Art. No. 4421).
Product Detail
5-Bromo-4-chloro-3-indolyl phosphate p -toluidine salt ≥99 %, BioScience Grade In combination with NBT ideal for colorimetric detection of alkaline phosphatase activity, in immunohistochemical procedures. Mechanism By enzymatic dephosphorylation BCIP is converted to indoxyl which subsequently tautomerizes to the ketone. In basic pH values, this ketone dimerizes and, in doing so, releases H + ions. The dye precipitates in the direct vicinity of the phosphatase, hence staining this site in a weak blue colour. Enhencement and darkening of this colour is achieved by adding NBT (Art. No. 4421).
Product Detail
p -Nitrotetrazolium blue chloride ≥98 %, p.a. Used in combination with BCIP-toluidine salt (X-phosphate) for detection of alkaline phosphatase activity by producing an indigo dye precipitate. Colour enhancer for staining with alkalic phosphatase. Mechanism During dimerisation of ketonized X-Phos, H + ions are set free which reduce NBT to purple-coloured diformazan. Both dyes precipitate in the direct vicinity of the phosphatase, hence staining this site in a dark violet. Store at +4 °C. Please also see assay protocol for use of BCIP/NBT in immunoblot procedures.
Product Detail
5-Bromo-4-chloro-3-indolyl phosphate disodium salt ≥99 % In combination with NBT ideal for colorimetric detection of alkaline phosphatase activity, in particular in immunoblot procedures.
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