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Chromatographically homogeneous preparation of Ribonuclease A (RNase A), free of DNase, protease, and salt. A major application for RNase A is the removal of RNA from preparations of plasmid DNA. It is also used in RNA sequence analysis, protection assays and removal of RNA contained in protein samples. RNase A is an endonuclease which specifically attacks pyrimidine sites (Py/pN) at the 3'-phosphate group. The highest activity is exhibited with single stranded RNA. Activators of RNase A include potassium and sodium salts. The enzyme is inhibited by heavy metal ions and competitively by DNA. Chromatographically homogeneous preparation of Ribonuclease A (RNase A), free of DNase, protease, and salt. A major application for RNase A is the removal of RNA from preparations of plasmid DNA. It is also used in RNA sequence analysis, protection assays and removal of RNA contained in protein samples. RNase A is an endonuclease which specifically attacks pyrimidine sites (Py/pN) at the 3'-phosphate group. The highest activity is exhibited with single stranded RNA. Activators of RNase A include potassium and sodium salts. The enzyme is inhibited by heavy metal ions and competitively by DNA.
Product Detail
RNase A content approx. 70 %.DNase-free RNase: To prepare RNase A free of DNase dissolve RNase A in TE buffer at 1 mg/ml and boil 10 to 30 minutes. Store aliquots at -20 °C to prevent microbial growth. RNase A content approx. 70 %.DNase-free RNase: To prepare RNase A free of DNase dissolve RNase A in TE buffer at 1 mg/ml and boil 10 to 30 minutes. Store aliquots at -20 °C to prevent microbial growth.
Product Detail
RNase A content approx. 70 %.DNase-free RNase: To prepare RNase A free of DNase dissolve RNase A in TE buffer at 1 mg/ml and boil 10 to 30 minutes. Store aliquots at -20 °C to prevent microbial growth. RNase A content approx. 70 %.DNase-free RNase: To prepare RNase A free of DNase dissolve RNase A in TE buffer at 1 mg/ml and boil 10 to 30 minutes. Store aliquots at -20 °C to prevent microbial growth.
Product Detail
Chromatographically homogeneous preparation of Ribonuclease A (RNase A), free of DNase, protease, and salt. A major application for RNase A is the removal of RNA from preparations of plasmid DNA. It is also used in RNA sequence analysis, protection assays and removal of RNA contained in protein samples. RNase A is an endonuclease which specifically attacks pyrimidine sites (Py/pN) at the 3'-phosphate group. The highest activity is exhibited with single stranded RNA. Activators of RNase A include potassium and sodium salts. The enzyme is inhibited by heavy metal ions and competitively by DNA. Chromatographically homogeneous preparation of Ribonuclease A (RNase A), free of DNase, protease, and salt. A major application for RNase A is the removal of RNA from preparations of plasmid DNA. It is also used in RNA sequence analysis, protection assays and removal of RNA contained in protein samples. RNase A is an endonuclease which specifically attacks pyrimidine sites (Py/pN) at the 3'-phosphate group. The highest activity is exhibited with single stranded RNA. Activators of RNase A include potassium and sodium salts. The enzyme is inhibited by heavy metal ions and competitively by DNA.
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
