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Iodonitrotetrazolium chloride, a tetrazolium dye also known as INT, serves as an electron acceptor for the colorimetric determination of various dehydrogenases, e.g. for the detection of LDH (1).For the colorimetric measurement of the enzymatic hydrolysis of the terminal galactose of GM1 ganglioside (2). INT forms a purple formazan dye during reduction. Iodonitrotetrazolium chloride, a tetrazolium dye also known as INT, serves as an electron acceptor for the colorimetric determination of various dehydrogenases, e.g. for the detection of LDH (1).For the colorimetric measurement of the enzymatic hydrolysis of the terminal galactose of GM1 ganglioside (2). INT forms a purple formazan dye during reduction.
Product Detail
Tetrazolium salt, abbreviated to MTT, is used for detection of dehydrogenases in combination with phenazine methosulfate (cat. no. 32030) (1). MTT is utilized as a colorimetric metabolic activity indicator in cell viability assays and to determine cell proliferation (3, 4). It works as a terminal electron acceptor in the cycling assay for pyridine nucleotides (2). MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. Tetrazolium salt, abbreviated to MTT, is used for detection of dehydrogenases in combination with phenazine methosulfate (cat. no. 32030) (1). MTT is utilized as a colorimetric metabolic activity indicator in cell viability assays and to determine cell proliferation (3, 4). It works as a terminal electron acceptor in the cycling assay for pyridine nucleotides (2). MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm.
Product Detail
The ditetrazolium compound nitro blue tetrazolium chloride, also known as NBT for short, serves as a substrate for the detection of succinate dehydrogenase (1), glucose-6-phosphate dehydrogenase (2) and xanthine oxidase (3). NBT is used in conjunction with BCIP (cat. no. 15247) for the colourimetric detection of alkaline phosphatase labelled molecules in Northern, Southern and Western blotting, in situ hybridisation and immunohistochemistry. When incubated with alkaline phosphatase, the BCIP/NBT substrate produces an insoluble, violet-stained NBT-diformazan precipitate that is easily detected on membranes or tissue sections.BCIP p-toluidine salt and NBT should be dissolved in dimethylformamide to prepare a stock solution.For added convenience, ready-to-use BCIP/NBT solutions are offered: SERVAColor BCIP/NBT Blot Solution, cat. no. 15245 and BCIP/NBT Ready-to-use Substrate for Immunohistochemistry and Blotting, cat. no. 15246.Stock solution for Western blots: 66 µl NBT stock solution and 33 µl BCIP stock solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris, pH 9.5). The ditetrazolium compound nitro blue tetrazolium chloride, also known as NBT for short, serves as a substrate for the detection of succinate dehydrogenase (1), glucose-6-phosphate dehydrogenase (2) and xanthine oxidase (3). NBT is used in conjunction with BCIP (cat. no. 15247) for the colourimetric detection of alkaline phosphatase labelled molecules in Northern, Southern and Western blotting, in situ hybridisation and immunohistochemistry. When incubated with alkaline phosphatase, the BCIP/NBT substrate produces an insoluble, violet-stained NBT-diformazan precipitate that is easily detected on membranes or tissue sections.BCIP p-toluidine salt and NBT should be dissolved in dimethylformamide to prepare a stock solution.For added convenience, ready-to-use BCIP/NBT solutions are offered: SERVAColor BCIP/NBT Blot Solution, cat. no. 15245 and BCIP/NBT Ready-to-use Substrate for Immunohistochemistry and Blotting, cat. no. 15246.Stock solution for Western blots: 66 µl NBT stock solution and 33 µl BCIP stock solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris, pH 9.5).
Product Detail
The ditetrazolium compound nitro blue tetrazolium chloride, also known as NBT for short, serves as a substrate for the detection of succinate dehydrogenase (1), glucose-6-phosphate dehydrogenase (2) and xanthine oxidase (3). NBT is used in conjunction with BCIP (cat. no. 15247) for the colourimetric detection of alkaline phosphatase labelled molecules in Northern, Southern and Western blotting, in situ hybridisation and immunohistochemistry. When incubated with alkaline phosphatase, the BCIP/NBT substrate produces an insoluble, violet-stained NBT-diformazan precipitate that is easily detected on membranes or tissue sections.BCIP p-toluidine salt and NBT should be dissolved in dimethylformamide to prepare a stock solution.For added convenience, ready-to-use BCIP/NBT solutions are offered: SERVAColor BCIP/NBT Blot Solution, cat. no. 15245 and BCIP/NBT Ready-to-use Substrate for Immunohistochemistry and Blotting, cat. no. 15246.Stock solution for Western blots: 66 µl NBT stock solution and 33 µl BCIP stock solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris, pH 9.5). The ditetrazolium compound nitro blue tetrazolium chloride, also known as NBT for short, serves as a substrate for the detection of succinate dehydrogenase (1), glucose-6-phosphate dehydrogenase (2) and xanthine oxidase (3). NBT is used in conjunction with BCIP (cat. no. 15247) for the colourimetric detection of alkaline phosphatase labelled molecules in Northern, Southern and Western blotting, in situ hybridisation and immunohistochemistry. When incubated with alkaline phosphatase, the BCIP/NBT substrate produces an insoluble, violet-stained NBT-diformazan precipitate that is easily detected on membranes or tissue sections.BCIP p-toluidine salt and NBT should be dissolved in dimethylformamide to prepare a stock solution.For added convenience, ready-to-use BCIP/NBT solutions are offered: SERVAColor BCIP/NBT Blot Solution, cat. no. 15245 and BCIP/NBT Ready-to-use Substrate for Immunohistochemistry and Blotting, cat. no. 15246.Stock solution for Western blots: 66 µl NBT stock solution and 33 µl BCIP stock solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris, pH 9.5).
Product Detail
Tetrazolium salt, abbreviated to MTT, is used for detection of dehydrogenases in combination with phenazine methosulfate (cat. no. 32030) (1). MTT is utilized as a colorimetric metabolic activity indicator in cell viability assays and to determine cell proliferation (3, 4). It works as a terminal electron acceptor in the cycling assay for pyridine nucleotides (2). MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. Tetrazolium salt, abbreviated to MTT, is used for detection of dehydrogenases in combination with phenazine methosulfate (cat. no. 32030) (1). MTT is utilized as a colorimetric metabolic activity indicator in cell viability assays and to determine cell proliferation (3, 4). It works as a terminal electron acceptor in the cycling assay for pyridine nucleotides (2). MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm.
Product Detail
The ditetrazolium compound nitro blue tetrazolium chloride, also known as NBT for short, serves as a substrate for the detection of succinate dehydrogenase (1), glucose-6-phosphate dehydrogenase (2) and xanthine oxidase (3). NBT is used in conjunction with BCIP (cat. no. 15247) for the colourimetric detection of alkaline phosphatase labelled molecules in Northern, Southern and Western blotting, in situ hybridisation and immunohistochemistry. When incubated with alkaline phosphatase, the BCIP/NBT substrate produces an insoluble, violet-stained NBT-diformazan precipitate that is easily detected on membranes or tissue sections.BCIP p-toluidine salt and NBT should be dissolved in dimethylformamide to prepare a stock solution.For added convenience, ready-to-use BCIP/NBT solutions are offered: SERVAColor BCIP/NBT Blot Solution, cat. no. 15245 and BCIP/NBT Ready-to-use Substrate for Immunohistochemistry and Blotting, cat. no. 15246.Stock solution for Western blots: 66 µl NBT stock solution and 33 µl BCIP stock solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris, pH 9.5). The ditetrazolium compound nitro blue tetrazolium chloride, also known as NBT for short, serves as a substrate for the detection of succinate dehydrogenase (1), glucose-6-phosphate dehydrogenase (2) and xanthine oxidase (3). NBT is used in conjunction with BCIP (cat. no. 15247) for the colourimetric detection of alkaline phosphatase labelled molecules in Northern, Southern and Western blotting, in situ hybridisation and immunohistochemistry. When incubated with alkaline phosphatase, the BCIP/NBT substrate produces an insoluble, violet-stained NBT-diformazan precipitate that is easily detected on membranes or tissue sections.BCIP p-toluidine salt and NBT should be dissolved in dimethylformamide to prepare a stock solution.For added convenience, ready-to-use BCIP/NBT solutions are offered: SERVAColor BCIP/NBT Blot Solution, cat. no. 15245 and BCIP/NBT Ready-to-use Substrate for Immunohistochemistry and Blotting, cat. no. 15246.Stock solution for Western blots: 66 µl NBT stock solution and 33 µl BCIP stock solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris, pH 9.5).
Product Detail
Tetrazolium salt, abbreviated to MTT, is used for detection of dehydrogenases in combination with phenazine methosulfate (cat. no. 32030) (1). MTT is utilized as a colorimetric metabolic activity indicator in cell viability assays and to determine cell proliferation (3, 4). It works as a terminal electron acceptor in the cycling assay for pyridine nucleotides (2). MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. Tetrazolium salt, abbreviated to MTT, is used for detection of dehydrogenases in combination with phenazine methosulfate (cat. no. 32030) (1). MTT is utilized as a colorimetric metabolic activity indicator in cell viability assays and to determine cell proliferation (3, 4). It works as a terminal electron acceptor in the cycling assay for pyridine nucleotides (2). MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm.
Product Detail
Triphenyltetrazolium chloride, abbreviated TTC, is used in biochemistry to measure the activity of dehydrogenases. In vital staining living cells are detected through the reduction of the colourless, water-soluble tetrazolium dye to a deep red, water-insoluble formazan via the dehydrogenases of the respiratory chain. Furthermore, TTC can be used in microbiology to distinguish between coliform and noncoliform bacteria, to measure the bacterial content of liquids in food analysis and to determine seed vitality. Triphenyltetrazolium chloride, abbreviated TTC, is used in biochemistry to measure the activity of dehydrogenases. In vital staining living cells are detected through the reduction of the colourless, water-soluble tetrazolium dye to a deep red, water-insoluble formazan via the dehydrogenases of the respiratory chain. Furthermore, TTC can be used in microbiology to distinguish between coliform and noncoliform bacteria, to measure the bacterial content of liquids in food analysis and to determine seed vitality.
Product Detail
Iodonitrotetrazolium chloride, a tetrazolium dye also known as INT, serves as an electron acceptor for the colorimetric determination of various dehydrogenases, e.g. for the detection of LDH (1).For the colorimetric measurement of the enzymatic hydrolysis of the terminal galactose of GM1 ganglioside (2). INT forms a purple formazan dye during reduction. Iodonitrotetrazolium chloride, a tetrazolium dye also known as INT, serves as an electron acceptor for the colorimetric determination of various dehydrogenases, e.g. for the detection of LDH (1).For the colorimetric measurement of the enzymatic hydrolysis of the terminal galactose of GM1 ganglioside (2). INT forms a purple formazan dye during reduction.
Product Detail
Monotetrazolium salt which forms a water-soluble formazan upon reduction. Suitable for anti-HIV (1 - 3) and anti-tumor (4 - 6) drug testing as well as for cell proliferation assays (7 - 8). Monotetrazolium salt which forms a water-soluble formazan upon reduction. Suitable for anti-HIV (1 - 3) and anti-tumor (4 - 6) drug testing as well as for cell proliferation assays (7 - 8).
Product Detail
Triphenyltetrazolium chloride, abbreviated TTC, is used in biochemistry to measure the activity of dehydrogenases. In vital staining living cells are detected through the reduction of the colourless, water-soluble tetrazolium dye to a deep red, water-insoluble formazan via the dehydrogenases of the respiratory chain. Furthermore, TTC can be used in microbiology to distinguish between coliform and noncoliform bacteria, to measure the bacterial content of liquids in food analysis and to determine seed vitality. Triphenyltetrazolium chloride, abbreviated TTC, is used in biochemistry to measure the activity of dehydrogenases. In vital staining living cells are detected through the reduction of the colourless, water-soluble tetrazolium dye to a deep red, water-insoluble formazan via the dehydrogenases of the respiratory chain. Furthermore, TTC can be used in microbiology to distinguish between coliform and noncoliform bacteria, to measure the bacterial content of liquids in food analysis and to determine seed vitality.
Product Detail
Tetrazolium salt, abbreviated to MTT, is used for detection of dehydrogenases in combination with phenazine methosulfate (cat. no. 32030) (1). MTT is utilized as a colorimetric metabolic activity indicator in cell viability assays and to determine cell proliferation (3, 4). It works as a terminal electron acceptor in the cycling assay for pyridine nucleotides (2). MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. Tetrazolium salt, abbreviated to MTT, is used for detection of dehydrogenases in combination with phenazine methosulfate (cat. no. 32030) (1). MTT is utilized as a colorimetric metabolic activity indicator in cell viability assays and to determine cell proliferation (3, 4). It works as a terminal electron acceptor in the cycling assay for pyridine nucleotides (2). MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm.
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
