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Sterile Rapid and simple test system for non-radioactive quantitation of proliferating cells. Also well suitable for cytotoxicity assays. Optimum replacement for MTT: Cells keep on being vital Most simple handling One-component-system without radioactivity For adherent and suspension cells Photometric result in 1 to 4 hours Strong correlation between absorbance and cell number Suitable for every culture media ROTITEST ® Vital ROTITEST ® BioAnalysis Grade, sterile, ready-to-use Mechanism Sensitivity of the tetrazolium salt used is higher than that known for standard reagents such as MTT, XTT, MTS, or WST-1. Results were shown to be of highly stringent correlation with [ 3 H]-thymidine incorporation assays, therefore representing viability as well as proliferation status of the cells (Tominaga et al. , Anal. Commun. 1999 (36) 47-50). In all living cells, NADH and NADPH are formed by the respiratory chain. ROTITEST ® vital is based on the (colourless) WST-8, which functions as acceptor for the NADH/NADPH dehydrogenase while being reduced to (coloured) formazane during the process. Directions for use The test solution is added directly to the cultivated cells in 96wells. During incubation for few hours, a water-soluble formazan dye is produced by viable cells, and is released into the surrounding medium. The resulting orange colour is measured photometrically (approx. 450 nm). Also suitable for culture media containing phenol red. Each package contains simple-to-follow instructions-for-use. Fig.: Example of use Fig. A: Correlation of cell counting results achieved with ROTITEST ® Vital (10 μl/well) and 3 H-Thymidine (37 KBq/well). HeLa (1, in DMEM) and HL60 cells (2, in MEM). Fig. B: Comparison of cell counting results using ROTITEST ® Vital (10 μl/well) and other cell counting reagents. Photometrical measurement at 450 nm (ROTITEST ® Vital, XTT), 490 nm (MTS), and 570 nm (MTT). HeLa (1, in DMEM) and HL60 cells (2, in MEM). Fig. C: Measurement of cytotoxic effects of actinomycin D (exposition 24 h) on HeLa cells (in DMEM), assayed with ROTITEST ® Vital (10 μl/well). Fig. D: Proliferation assay on CTLL-2 cells (in RPMI) under influence of humane IL-2 (exposition 72 h), using ROTITEST ® Vital (10 μl/well).
Product Detail
As an anionic azo dye trypan blue binds to the cell proteins. Since it is not membrane-permeable, it only stains dead cells. Directions for use It is commonly used in a 0,4 % aqueous solution to determine the vitality of cells. The solution is sterile-filtered and durable for a period of two years. Trypan blue solution 0.4 % 0,4 %, for microscopy
Product Detail
Sterile Rapid and simple test system for non-radioactive quantitation of proliferating cells. Also well suitable for cytotoxicity assays. Optimum replacement for MTT: Cells keep on being vital Most simple handling One-component-system without radioactivity For adherent and suspension cells Photometric result in 1 to 4 hours Strong correlation between absorbance and cell number Suitable for every culture media ROTITEST ® Vital ROTITEST ® BioAnalysis Grade, sterile, ready-to-use Mechanism Sensitivity of the tetrazolium salt used is higher than that known for standard reagents such as MTT, XTT, MTS, or WST-1. Results were shown to be of highly stringent correlation with [ 3 H]-thymidine incorporation assays, therefore representing viability as well as proliferation status of the cells (Tominaga et al. , Anal. Commun. 1999 (36) 47-50). In all living cells, NADH and NADPH are formed by the respiratory chain. ROTITEST ® vital is based on the (colourless) WST-8, which functions as acceptor for the NADH/NADPH dehydrogenase while being reduced to (coloured) formazane during the process. Directions for use The test solution is added directly to the cultivated cells in 96wells. During incubation for few hours, a water-soluble formazan dye is produced by viable cells, and is released into the surrounding medium. The resulting orange colour is measured photometrically (approx. 450 nm). Also suitable for culture media containing phenol red. Each package contains simple-to-follow instructions-for-use. Fig.: Example of use Fig. A: Correlation of cell counting results achieved with ROTITEST ® Vital (10 μl/well) and 3 H-Thymidine (37 KBq/well). HeLa (1, in DMEM) and HL60 cells (2, in MEM). Fig. B: Comparison of cell counting results using ROTITEST ® Vital (10 μl/well) and other cell counting reagents. Photometrical measurement at 450 nm (ROTITEST ® Vital, XTT), 490 nm (MTS), and 570 nm (MTT). HeLa (1, in DMEM) and HL60 cells (2, in MEM). Fig. C: Measurement of cytotoxic effects of actinomycin D (exposition 24 h) on HeLa cells (in DMEM), assayed with ROTITEST ® Vital (10 μl/well). Fig. D: Proliferation assay on CTLL-2 cells (in RPMI) under influence of humane IL-2 (exposition 72 h), using ROTITEST ® Vital (10 μl/well).
Product Detail
Sterile Rapid and simple test system for non-radioactive quantitation of proliferating cells. Also well suitable for cytotoxicity assays. Optimum replacement for MTT: Cells keep on being vital Most simple handling One-component-system without radioactivity For adherent and suspension cells Photometric result in 1 to 4 hours Strong correlation between absorbance and cell number Suitable for every culture media ROTITEST ® Vital ROTITEST ® BioAnalysis Grade, sterile, ready-to-use Mechanism Sensitivity of the tetrazolium salt used is higher than that known for standard reagents such as MTT, XTT, MTS, or WST-1. Results were shown to be of highly stringent correlation with [ 3 H]-thymidine incorporation assays, therefore representing viability as well as proliferation status of the cells (Tominaga et al. , Anal. Commun. 1999 (36) 47-50). In all living cells, NADH and NADPH are formed by the respiratory chain. ROTITEST ® vital is based on the (colourless) WST-8, which functions as acceptor for the NADH/NADPH dehydrogenase while being reduced to (coloured) formazane during the process. Directions for use The test solution is added directly to the cultivated cells in 96wells. During incubation for few hours, a water-soluble formazan dye is produced by viable cells, and is released into the surrounding medium. The resulting orange colour is measured photometrically (approx. 450 nm). Also suitable for culture media containing phenol red. Each package contains simple-to-follow instructions-for-use. Fig.: Example of use Fig. A: Correlation of cell counting results achieved with ROTITEST ® Vital (10 μl/well) and 3 H-Thymidine (37 KBq/well). HeLa (1, in DMEM) and HL60 cells (2, in MEM). Fig. B: Comparison of cell counting results using ROTITEST ® Vital (10 μl/well) and other cell counting reagents. Photometrical measurement at 450 nm (ROTITEST ® Vital, XTT), 490 nm (MTS), and 570 nm (MTT). HeLa (1, in DMEM) and HL60 cells (2, in MEM). Fig. C: Measurement of cytotoxic effects of actinomycin D (exposition 24 h) on HeLa cells (in DMEM), assayed with ROTITEST ® Vital (10 μl/well). Fig. D: Proliferation assay on CTLL-2 cells (in RPMI) under influence of humane IL-2 (exposition 72 h), using ROTITEST ® Vital (10 μl/well).
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
