Sterile ROTI ® Transform is a simple, effective and fast method of producing competent E. coli in three steps. The competent cells may be used directly for transformation or may be frozen for later transformations. Flexible selection of strains Effective, simple and fast Adequate for 500 transformations Transformation efficiency is 5 x 10 5 to 1 x 10 6 colonies per µg DNA. ROTI ® Transform sterile, ready-to-use Directions for use Protocol: 1. Resuspend cells in fresh, cold LB-medium 2. Add ROTI ® Transform 1 3. Add ROTI ® Transform 2 and incubate for 5 minutes on ice. The kit contains ROTI ® Transform consists of 45 ml ROTI ® Transform 1 (Art. No. P041) and 5 ml ROTI ® Transform 2 (Art. No. P042). User information Long-term Storage of the Kit at -20 °C. Storage for use: Reagent 1: Short-term storage may be done at 4 °C. For long-term storage of over 14 days we recommend a storage temperature of -20 °C. Reagent 2: Storage at 4 °C.