Material: borosilicate glass 3.3. In thin-layer chromatography, the separation of the components of a mixture is the most delicate step, as it is subject to different and variable parameters. Some are perfectly controlled (chemical nature of the stationary and mobile phase), others are more random (degree of humidity, ambient temperature, pre-saturation). All require precise and rigorous protocols to achieve correct, repeatable and reproducible separations. The quality of the migration separation chamber and the choice of its size and shape are very important. Further attractive products to complete your chromatography laboratory can be found on our Chromatography page! Twin Trough Chamber for TLC biostep ® (DESAGA) The double trough chamber with flat glass lid makes it possible to separate the TLC plate to be developed from the filter paper in order to saturate the atmosphere and at the same time save reagents, as reagent uptake is reduced to a minimum. Welded side walls Smooth glass lid seals