Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort. ROTI ® Garose-His uncharged HPBeads offer the perfect solution for your specific application under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified. Easy elution and regeneration Very low metal leaching Compatible with denaturing reagents The matrix of ROTI ® Garose-His uncharged HPBeads consists of crosslinked and beaded 6 % agarose, IDA-conjugated. In one step, the beads can be loaded safely and rapidly with all standard bivalent metal cations (Ni 2+ , Co 2+ , Zn 2+ , Cu 2+ ). ROTI ® Garose-His uncharged HPBeads result in eluates with considerably low metal contamination. Directions for use The tridentate IDA cross-linker provides easy elution with low amounts of imidazole. Additionally, ROTI ® Garose-His uncharged HPBeads may repeatedly be regenerated, making them very cost-effective. The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride, 5 mM DTT). ROTI ® Garose-His uncharged HPBeads ROTI ® Garose for biochemistry Uncharged IDA-agarose beads for affinity chromatography under medium pressure, with high flow rate or with big sample-/matrix volume. In ROTI ® Garose-His uncharged HPBeads, the advantages of the efficient metal ion/His binding have been combined with a bead technology allowing a very rapid flow rate. 50 % bead suspension in 20 % ethanol. Rapid one-step purification of His-tagged proteins from total lysates High binding capacity for standard me 2+ ions under rapid one-step loading For batch mode, gravity flow, MPLC und FPLC Recommended for big matrix volumes Suitable for Suitable for MPLC and FPLC, or if His-tag proteins shall be isolated either in particularly short time or in big amounts. Directions for use May be autoclaved at 121 °C for 30 mins.