Isopropanol as Clearing Agent Isopropanol is well suited as clearing agent for paraffin-embedding, because it is compatible to melted paraffin: After dehydrating with isopropanol the sample is incubated in a mixture of isopropanol abs. and paraffin (1:1) at approx. 60 °C. After a further incubation step with pure paraffin, the sample is embedded as usual. This method is particularly suitable to smooth organs. Since embedding can start immediately after alcohol step, the working process is shortened significantly. For easy opening of the ROTITAINER ® we recommend the barrel and keg key Tri-Sure ® (Art. No. 0398.1) from ROTH SELECTION. Video instructions can be found here and above in the product pictures. 2-Propanol ROTIPURAN ® ≥99,8 %, p.a., ACS, ISO