In PBS.Demecolcine depolymerizes microtubules and limits microtubule formation. It inactivates spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate. Induces apoptosis by blocking mitosis in HeLa S3 cells. The mechanism of action is similar to that of colchicine, but with lower mammalian toxicity. In PBS.Demecolcine depolymerizes microtubules and limits microtubule formation. It inactivates spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate. Induces apoptosis by blocking mitosis in HeLa S3 cells. The mechanism of action is similar to that of colchicine, but with lower mammalian toxicity.