For cultivation of E. coli in molecular biology.10 g/l Tryptone5 g/l Yeast extract5 g/l NaClFor making 1 L liquid medium suspend 20 g in 900 ml distilled water, adjust the pH to 7.0 with approximately 0.2 ml of 5 N NaOH, fill up to a final volume of 1 L with deionized water and sterilize by autoclaving. For cultivation of E. coli in molecular biology.10 g/l Tryptone5 g/l Yeast extract5 g/l NaClFor making 1 L liquid medium suspend 20 g in 900 ml distilled water, adjust the pH to 7.0 with approximately 0.2 ml of 5 N NaOH, fill up to a final volume of 1 L with deionized water and sterilize by autoclaving.