Isolated from Baker's yeast by recombinant DNA technology. Identical in structure and function to the native human sequence. Essential for long-term growth of various cell lines. Stimulates the proliferation of cells and supports carbohydrate metabolism. Absence of insulin in the medium may result in disturbances of cell morphology and growth rate. Recommended concentration for use in serum free media is 1 – 10 µg/ml. Isolated from Baker's yeast by recombinant DNA technology. Identical in structure and function to the native human sequence. Essential for long-term growth of various cell lines. Stimulates the proliferation of cells and supports carbohydrate metabolism. Absence of insulin in the medium may result in disturbances of cell morphology and growth rate. Recommended concentration for use in serum free media is 1 – 10 µg/ml.