Irreversible inhibitor of thrombin and other serine proteases (e.g. chymotrypsin, kallikrein, plasmin, proteinase K, trypsin). Inhibits by acylation of the active site of the enzyme. AEBSF is water-soluble and much less toxic than PMSF and DFP. Aqueous solutions are stable between pH 5 - 6, limited stability above pH 7.5. Irreversible inhibitor of thrombin and other serine proteases (e.g. chymotrypsin, kallikrein, plasmin, proteinase K, trypsin). Inhibits by acylation of the active site of the enzyme. AEBSF is water-soluble and much less toxic than PMSF and DFP. Aqueous solutions are stable between pH 5 - 6, limited stability above pH 7.5.