Discontinuous buffer system for optimum transfer in the semi-dry blotter. Optimised for semi dry blotting Superior blotting results for peptides and proteins of every size Special formulation with reduced acid formation - for optimal electrode protection In the semi-dry blotting system, both a continuous buffer system (identical buffer at anode and cathode) and a discontinuous buffer system (different buffer at anode and cathode) can be used. The transfer performance of discontinuous systems is, however, generally higher, since the two buffers are designed separately according to the needs of the two electrodes. The discontinuous buffer system ROTI ® Blot 1 consists of two different buffers, the anode buffer A (pH 7.8 ±0.1) and the cathode buffer K (pH 8.5 ±0.1). ROTI ® Blot 1 shows a much better transfer efficiency than a tris-glycine buffer and can be used for proteins and peptides of any length. Directions for use The blot stack is built up by soaking the upper blotting papers with cathode buffer and the lower blotting papers with anode buffer. With slightly longer transfer times, proteins >100 kDa can also be completely transferred with ROTI ® Blot 1. A detailed instruction manual is enclosed with the product. ROTI ® Blot K 10x conc., for electrophoresis