Used to prepare citrate buffer for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links, thus unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, and resulting in enhanced staining intensity of antibodies. It has an anticoagulant activity and forms as a calcium chelator complexes that disrupt the tendency of blood to clot. Used to prepare citrate buffer for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links, thus unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, and resulting in enhanced staining intensity of antibodies. It has an anticoagulant activity and forms as a calcium chelator complexes that disrupt the tendency of blood to clot.