The Snapwell insert is a modified Transwell culture insert that contains a 12 mm diameter tissue culture-treated membrane supported by a detachable ring. The inserts are primarily used for transport and electrophysiological studies. Once cells are grown to confluence, this ring-supported membrane can be placed into either vertical or horizontal diffusion or Ussing chambers. The Snapwell insert is a modified Transwell culture insert that contains a 12 mm diameter tissue culture-treated membrane supported by a detachable ring. The inserts are primarily used for transport and electrophysiological studies. Once cells are grown to confluence, this ring-supported membrane can be placed into either vertical or horizontal diffusion or Ussing chambers.