Sterile Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from mammalian blood by density gradient centrifugation. Density 1, 077 g/ml. Best alternative to Ficoll ® . Optimised for isolation of lymphocytes from non-coagulated mammaliam whole blood Produces distinct, compact layers Results in high recovery rates of viable cells Maintains the representative ratio of B- to T lymphocytes May be directly applied to all protocols using Ficoll ® ROTI ® Sep 1077 animal excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ration of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range. Suitable for application with non-coagulated peripheral whole blood from all mammals. In all respective protocols originally using Ficoll ® this reagent may be replaced by ROTI ® Sep 1077 animal without any adjustment. ROTI ® Sep 1077 animal CELLPURE ® sterile, ready-to-use, for density gradient centrifugation Not a medical device / Not an IVD product