ROTI ® Fect RNAi Kit CELLPURE ® ready-to-use, for transfection New, powerful transfection reagent for liposoma mediated siRNA and miRNA transfection of all mammalian cells in culture. Liposoma formulation and enclosed buffer were specially established and designed for core parameters of RNAi: Most efficient quantitative endocytosis plus release of nucleic acids into the cytosol, without subsequent transport to the nucleus. ROTI ® Fect RNAi provides you with superior knockdown results, even using low amounts of RNA.Hereby, the ROTI ® Fect technology guarantees minimisation of toxic effects during transfection, thus making these reagents suitable for very sensitive cell lines of primary cells. The detailed instruction-for-use contains a standardised transfection protocol working via a definite RNA/liposoma ratio and providing good results starting with the very first assay - making elaborate optimisations of transfection obsolete. Evaluation of results is often possible after 48 hours, enabling two sequential assays per week. Optimised RNA release into the cytosol High levels of gene silencing with low RNA concentration Efficient knockdown after 48 hours already No elaborate optimisation necessary Very low cell toxicity Suitable for cell lines and primary cells Straightforward, efficient protocol for whole medium included Successfully tested on a variety of cell types. Primary cells: MCS, M1 Cell lines: A549, 3T3-L1, B16F10, CHO-K1, H441, HT1080, HeLa, HeLa-KB, HepG2 The kit contains: 0.2 and 1 ml, respectively, ROTI ® Fect RNAi liposoma formulation (Art. No. 2911), and 0.4 and 2 ml, respectively, ROTI ® Fect RNAi buffer (Art. No. 2913). Les composants unitaires de ce kit ne peuvent pas être achetés séparément. Approx. 650 assays per ml in 24wells. Dependend on the cell line used. Figure: Knockdown of luciferase activity in stably Luc-transfected cell lines and cells (48 wells, 2 × 10 4 cells, 30 pMol siRNA). Percent reduction in luciferase activity (measured by luminometer) in knockdown cells compared to cells transfected with control RNA. Measurement 2 days after transfection. Figure: Knockdown of luciferase activity in stably Luc-transfected cell lines and cells (48 wells, 2 × 10 4 cells, 30 pMol siRNA). Percent reduction in luciferase activity (measured by luminometer) in knockdown cells compared to cells transfected with control RNA. Comparison of results obtained using different transfection reagents. Measurement 2 days after transfection.