ROTI ® Prep gDNA Mini 2.0 for molecular biology ROTI ® Prep gDNA MINI 2.0 was developed to isolate genomic DNA from a wide variety of starting materials. The isolation is based on a new procedure and combines sample lysis with the subsequent binding of the DNA to the surface of a column membrane. After several washing and centrifugation steps, the DNA is eluted from the membrane using an elution buffer. The extraction procedure in this kit is optimised to achieve maximum yield. For DNA extraction from bacteria, fungi and eukaryotic cell, blood and tissue samples. Preparation in the well-established mini-column system Fast, simple and reliable Extraction time of approx. 8 min (without lysis step) Yield, depending on the starting material, of up to 65 µg gDNA Possible basic material: Microbial cell cultures (max. 5x10 10 ) Fungi and eukaryotic cell cultures (max. 5x10 6 ) Rodent tail (0.5-1 cm) Oral mucosal swabs Tissue samples Blood (200-400 µl) The kit contains: Lysis Buffer LSV, Lysis Buffer LSN, Binding Buffer BR, Binding Buffer BSN, Proteinase K, RNase A (10 mg/ml), Washing Buffer WSO, Washing Buffer WSH (conc.), Washing Buffer WST (conc.), Elution Buffer EB, Centrifugation columns, Collection tubes, Elution tubes, detailed instructions. Ethanol 99.8 % (p.a., Art. No. 9065), RNase free water (Art. No. 1HPE.1), TE buffer (order no. 1052.1) and PBS (Art. No. 1058.1) are not included in the kit and should be ordered separately if required. Not a medical device / Not an IVD product