ROTI ® Prep Genomic DNA MINI for molecular biology Roti ® Prep Genomic DNA MINI has been developed for the isolation of genomic DNA from various sources. The isolation is carried out through the proven and reliable spin column technique, which is very easy to handle and needs only few steps. Preparation by well-known mini spin-column system Fast, easy and efficient Preparation in approx. 15 minutes (excluding lysis) Yield up to 65 μg gDNA, depending on source material In addition to the especially on proteinase K adapted lysis buffer, the filtration spin column has been optimised, permitting particularly high extraction rates. Extraction from up to 40 mg tissue, therefore, allows a yield of up to μg µg DNA. The thereby extracted DNA is free of RNA and proteins, and can directly be stored or used for downstream applications. Possible basic material: Tissue (up to 40 mg) Rodent tails (0,5-1 cm) FFPE tissue (formalin-fixed, paraffin-embedded) Buccal swabs Cell culture (up to 5x10 6 cells) Figure: Typical test gel following isolation of genomic DNA (A) and subsequent GAPDH-PCR (B) from rodent tail (frozen, 8 mm each). Yield of gDNA (from left to right): 22,6 μg, 27,7 μg and 28 μg. Ratio A 260 :A 280 = 1,97±0,01. The kit contains: Lysis Buffer LSA, Binding Buffer BSN, Proteinase K, Washing Buffer WST, Elution Buffer EB, spin columns, collection tubes, elution tubes, detailed instructions-for-use. Contents of this kit may not be bought separately. Please order ethanol 99,8 % (p.a., Art. No. 9065), RNase A (Art. No. 7156, not for FFPE tissue), and for FFPE tissue xylole (Art. No. 4436) or octane separately. Not a medical device / Not an IVD product