ROTI ® Prep Plasmid MINI-XL for molecular biology New - With optimised protocol for isolation from 0.5-2.0 ml bacterial culture ! MIDI preps in MINI size-fomat. ROTI ® Prep Plasmid MINI-XL is a further development of the classical Plasmid Mini extraction kit, which is based on an alkaline lysis and binding of the plasmid to the spin filter. The binding capacity of the spin filter has been, compared to usual plasmid mini kits, considerably increased (approx. 80 μg plasmid DNA) and allows now a plasmid extraction from 0.5-15 ml bacteria culture. Since filter dimensions correspond to the usual miniprep spin filters, its application differs only slightly from other plasmid mini kits. Most significant difference is the afore done convenient lysis in a 15 ml tube. Preparation by well-known mini spin-column system Fast, easy and efficient Preparation from 0.5-15 ml bacteria culture Preparation within approx. 20 minutes Yield 40-60μg (average per 15 ml bacterial culture). The elution resulting in highly concentrated pure DNA in 100 μl, takes then place in a 1,5 ml tube, like in standard mini kits. Isolated DNA is free of RNA or protein contamination and can be directly applied to all standard down-stream applications like restriction digest, sequencing, in vitro transcription, transfection etc. The kit contains: Resuspension Buffer RB, Lysis Buffer LS, Neutralisation Buffer NB, Washing Buffer PA, Washing Solution PB, Elution Buffer XEB, spin columns, collection tubes, elution tubes, detailed instructions-for-use. Contents of this kit may not be bought separately. Please order ethanol 96-99,8 % (p.a., Art. No. 9065) separately.