For cultivation of E. coli in molecular biology.10 g/l Tryptone5 g/l Yeast extract5 g/l NaCl15 g/l AgarFor making 1 L liquid medium suspend 35 g in 900 ml distilled water, adjust the pH to 7.0 with approximately 0.2 ml of 5 N NaOH, fill up to a final volume of 1 L with deionized water and sterilize by autoclaving. Cool to 45 °C prior to dispensing into sterile petri dishes. For cultivation of E. coli in molecular biology.10 g/l Tryptone5 g/l Yeast extract5 g/l NaCl15 g/l AgarFor making 1 L liquid medium suspend 35 g in 900 ml distilled water, adjust the pH to 7.0 with approximately 0.2 ml of 5 N NaOH, fill up to a final volume of 1 L with deionized water and sterilize by autoclaving. Cool to 45 °C prior to dispensing into sterile petri dishes.