Mixture of at least 10 proteases: five serine type proteases, two zinc endopeptidases, two zinc leucine aminopeptidases and one zinc carboxypeptidase. Digestion with the product has been useful when extensive or complete degradation of protein is required. Pronase E is used in tissue dissociation of various tissues, e.g. to isolate living chondrocytes.Additional applications are the structural analysis of proteins (1, 2), preparation of bacteriophage lambda DNA (3), pretreatment of tissue sections to enhance the intensity of immunostaining and removal of protein in DNA/RNA isolations. Mixture of at least 10 proteases: five serine type proteases, two zinc endopeptidases, two zinc leucine aminopeptidases and one zinc carboxypeptidase. Digestion with the product has been useful when extensive or complete degradation of protein is required. Pronase E is used in tissue dissociation of various tissues, e.g. to isolate living chondrocytes.Additional applications are the structural analysis of proteins (1, 2), preparation of bacteriophage lambda DNA (3), pretreatment of tissue sections to enhance the intensity of immunostaining and removal of protein in DNA/RNA isolations.