Deoxyribonuclease I, or DNase I • is used to nick DNA as a first step to incorporate labelled bases into DNA • is suitable for DNase footprinting • improves yield in primary cell isolation by removing extracellular DNA • reduces viscosity in protein samples by removal of DNA contaminationDNase I is an endonuclease that cleaves phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5'-phosphates. It preferentially cleaves double-stranded DNA and, in the presence of Mg2+ , single-stranded DNA. Deoxyribonuclease I, or DNase I • is used to nick DNA as a first step to incorporate labelled bases into DNA • is suitable for DNase footprinting • improves yield in primary cell isolation by removing extracellular DNA • reduces viscosity in protein samples by removal of DNA contaminationDNase I is an endonuclease that cleaves phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5'-phosphates. It preferentially cleaves double-stranded DNA and, in the presence of Mg2+ , single-stranded DNA.