Hydrolase belonging to the Amidases. Metallo-enzyme. Urease hydrolasis urea, resulting in ammonia and carbon dioxide, or in ammonia- and carbonate ions, respectively. Using high amounts of urea as substrate (200 mM), urease is applied for visualisation of the connection between reaction rate and time. Detection is carried out via measurement of conductivity, or, photometrically, via colour change of bromothymol blue. Furthermore, model system for catalytic activation of carbon dioxide in metallo-organic chemistry. Directions for use pH optimum: 7.4. Recommended reaction buffer system is 40 mM potassium phosphate buffer, pH 7.4. Heavy metals (e. g. lead) are inhibitory. Urease ≥220 Nessler U/mg material, lyophilized Unit definition One unit (U) is defined as the amount of enzyme that catalyzes the liberation of 1 µmole of NH 3 per min at pH 7.0 and 25 °C. Not a medical device / Not an IVD product