A pancreatic serine protease with a specificity for peptide bonds between basic amino acids arginine or lysine and another non-specific amino acid. Trypsin is frequently used to suspend cells which grow as monolayers on surfaces. Longer exposure to trypsin can damage cells, so that an inhibitor (serum or trypsin inhibitor from soya beans) should be added upon completion. Please also check our ready-to-use trypsin solutions for this purpose. Unit definition One USP Unit will cause an increase in absorption (253 nm) per minute of 0.003 at 25 °C and pH 7.6 using BAEE as substrate. One (international) Unit (U, 255 nm, BAEE, pH 8) is approx. 90 USP U. One TAME Unit is approx. 20 USP Units. One NF Unit is one USP Unit. Ten „BAEE Units“ are approx. 3.3 USP Units. Directions for use pH optimum 7.2-8.5 Trypsin ≥2500 USP-U/mg, lyophilized