The TBE buffer is optimally suited as a gel and running buffer system. Mechanism TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels. ROTIPHORESE ® TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels. ROTIPHORESE ® 5x TBE Buffer ROTIPHORESE ® 5x conc. ROTIPHORESE ® 5x TBE buffer contains 0,5 M Tris-Borate pH 8,3 and 10 mM EDTA in distilled, deionised water; pH 8,3. Suitable for manufacturing sequencing gels with ROTIPHORESE ® Gel 40 and as a running buffer. Directions for use ROTIPHORESE ® TBE buffer is usually used as 1x or 0.5-fold concentrated solution. TBE buffer is particularly suitable for the separation of small DNA fragments in the gel (up to 3 kb). TBE buffer is not suitable for use of gels for gel extraction of DNA. Mechanism TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.