The TBE buffer is optimally suited as a gel and running buffer system. Mechanism TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels. ROTIPHORESE ® TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels. ROTIPHORESE ® NF 10x TBE Buffer ROTIPHORESE ® 10x conc., fluorescence-free 10-times concentrated buffer formulated specially for automated sequencing. 10x stock solution contains 0,89 M Tris-Borate and 22 mM EDTA in distilled, deionised water; pH 8,3. Directions for use Suitable for manufacturing sequencing gels with ROTIPHORESE ® NF Acrylamide/Bis-solution 40 % (19:1) and as running buffer.In sequencing, NF-TBE buffer is usually used in a final concentration of 1x. Ideally suited as a gel and running buffer system and also for sequencing gels,