Loading bufer with the fluorescent dye SYBR ® Green I for staining of double stranded nucleic acid in agarose and polyacrylamide gels. Alternative for ethidium bromide, non-toxic, non-mutagen 20 times more sensitive than ethidium bromide, up to 0.01 ng nucleic acid per band For use with common broad-band ethidium bromide photo filters Excitation possible via UV light (254 nm) and blue light (495 nm) Compatible with all usual down-stream applications. ROTI ® Load DNAstain SYBR ® Green is easy to use and reduces significantly the time required for gel analysis. The contained fluorescent dye SYBR ® Green I detects up to 0.01 ng per band, and is, therefore, 20 times more sensitive than ethidium bromide. DNA integrity is not influenced by SYBR ® Green I; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligation, PCR, transformation, transfection etc. without interfering effects. SYBR ® Green I bounds to the minor groove of DNA and intercalates into the DNA. It can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE ® PROfessional runVIEW, Art. No. 4849.1). When excited, nucleic acid-bound SYBR ® Green I emits a brightly coloured green fluorescence (521 nm) that may be documented by all usual broadband ethidium bromide- or SYBR ® Green foto filters. The figure depicts 50 ng DNA per band, or 75 ng (1000 bp and 2.5 kb), respectively. Each lane has been loaded and concurrently stained using 1 µl ROTI ® Load DNAstain SYBR ® Green. Wavelength Excitation maximum (bound to DNA): 254 nm and 495 nm Emission maximum (bound to DNA): 521 nm ROTI ® Load DNAstain 1 Green 6x conc., ready-to-use Directions for use In order to cover the main size range needed in gel electrophoresis, ROTI ® Load DNAstain 1 Green contains bromophenol blue and xylene cyanol. Glycerin is the standard reagent for increasing sample density. Recommended for staining of dsDNA- and dsRNA-fragments of over 500 bp.