The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly. ROTI ® Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose. Working concentration ROTI ® Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI ® Load DNA. ROTI ® Load DNA gel loading buffer is usually used as 1x concentrated solution. ROTI ® Load DNA orange 2 (with glycerol) 6x conc., DNase-free Directions for use ROTI ® Load DNA-orange 2 (with glycerol) is a gel loading buffer with orange G and xylencyanol as dyes. Orange G runs in the very small fragment range and shows the maximum running distance for very short runs (see lane 5 in the product image). Well suited if a large fragment range is to be separated in the gel or if bromophenol blue could mask important DNA bands. Glycerin is the standard reagent for increasing sample density. Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.