The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly. ROTI ® Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose. Working concentration ROTI ® Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI ® Load DNA. ROTI ® Load DNA gel loading buffer is usually used as 1x concentrated solution. ROTI ® Load DNA 1x (with glycerol) 1x conc., ready-to-use, DNase-free Directions for use In order to cover the main size reange needed in gel electrophoresis, ROTI ® Load DNA 1x (with glycerol) contains bromophenol blue and xylene cyanol (see lane 1 in the product image). Glycerin is the standard reagent for increasing sample density. Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.