The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly. ROTI ® Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose. Working concentration ROTI ® Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI ® Load DNA. ROTI ® Load DNA gel loading buffer is usually used as 1x concentrated solution. ROTI ® Load DNA short-run 1x (with glycerol) 1x conc., ready-to-use, DNase-free Directions for use In order to prevent important DNA bands being clouded, ROTI ® Load DNA short-run 1x (with glycerol) contains only bromophenol blue in slightly reduced concentration (see lane 2 in the product image). The buffer may also be diluted to 0,6x if the bromophenol blue in the gel could mask an important DNA band. Glycerin is the standard reagent for increasing sample density. Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.