The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly. ROTI ® Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose. Working concentration ROTI ® Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI ® Load DNA. ROTI ® Load DNA gel loading buffer is usually used as 1x concentrated solution. ROTI ® Load DNA (with ficoll) 6x conc., DNase-free Directions for use In order to cover the main size reange needed in gel electrophoresis, ROTI ® Load DNA (with ficoll) contains bromophenol blue and xylene cyanol (see lane 1 in the product image). Ficoll ® 400 produces particularly sharp bands and is often used when smaller amounts of DNA are to be depicted in the gel. Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.