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Kovacs’ Reagent

£ 63.35*

*Prices plus VAT plus shipping costs

£ 76.02*

*incl. VAT, plus shipping costs

Kovacs’ Reagent

Order number: 2950.1

Price Breakdown

24

6

1

Production Description

Kovacs’ Reagent for microbiology Tryptophanase-positive microorganisms (e.g. E. coli, S. faecalis, Proteus sp. ) that cleave tryptophane hereby release indole, which may be detected by reaction with dimethylamino benzaldehyde by forming a dark red dye. Microorganisms must be incubated in a glucose-free broth with high concentrations of tryptophane present. Kovacs’ Reagent is added to 24-48 h old cultures in a concentration of 0.2 to 0.5 ml per 5 ml broth. A colour shift to dark red shows presence of indole, thus confirming the inoculating colony as being indole-/tryptophanase-positive.

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