Material: PP columns with PE filter elements. Further attractive products to complete your chromatography laboratory can be found on our Chromatography page! Eluents with higher alcohol contents cause an increase in volume of the aqueous phase on the CHROMABOND ® XTR. In such case, the column might be overloaded which leads to a displacement of the aqueous phase from the column. Therefore a greater capacity column should be used. Extraction of analytes form an aqueous to an organic phase: Column conditioning: Not required Sample application: Aqueous solutions are applied to the dry CHROMABOND ® XTR adsorbent. They are soaked up by the solid within a few minutes and spread over the surface of the kieselguhr material as a thin film. Elution: Lipophilic analytes are eluted with water-immiscible organic solvents; the aqueous phase remains on the CHROMABOND ® XTR adsorbent. Polar, water-soluble analytes, which remain in the aqueous phase on the XTR adsorbent, can be eluted e.g. with saturated NaCl. Warning: Never exceed the volume capacities listed for each column size! Solvents applicable for elution: Diethyl ether (T900) tert -Butyl methyl ether (T175) Ethyl acetate (T164) n -Hexane (T165) Cyclohexane (T163) Toluene (T166) Dichloromethane (T901) Trichloromethane (chloroform) (T901) Trichloromethane/methanol (90/10 or 85/15, v/v) (T901/T909) Diethyl ether/ethanol (90/10 or 80/20, v/v) (T900/9065) Dichloromethane/2-propanol (90/10 or 85/15, v/v) (T901/T902) General column parameters: Depending on the concentration of the analytes eluates can be analysed immediately, or the organic solvent is evaporated. The pH value of the aqueous solution can be altered on the column, which enables elution of different compounds of a sample under optimised conditions. Under certain circumstances, acidic, neutral, and basic compounds can be fractionated in this way. Volume Filling quantity Max. Loadability with aqueous solution Waiting time before elution Elution volume 6 ml 1 g 1 ml 5-10 min 8 ml 30 ml 4,5 g 5 ml 5-10 min 16 ml 70 ml 14,5 g 20 ml 10-15 min 40 ml Polypropylene columns CHROMABOND ® XTR Kieselguhr phase for liquid-liquid extraction Base material coarse-grained kieselguhr (also known as diatomaceous earth, hydromatrix, celite ® ) Application: Liquid-liquid extraction of highly viscous aqueous solutions such as physiological fluids (blood, plasma, and serum), dyes in textiles, environmental and food analysis without use of a separation funnel High water loadability without breakthrough of water during elution with organic solvents Also suitable for removing small amounts of water from solvents which are not miscible with water pH stability: 1-13 (1) Sample application (2) Adsorption of the sample (3) Elution of the sample Advantages: Fast, reproducible and economical Simultaneous preparation of several samples No problems with phase separation, no formation of emulsions High recovery rates Saves of time and solvents Organic solutions don‘t need to be dried after separation Large pore size, high pore volume, constantly high batch-to-batch quality Similar phases: EXtrelut ® , Chem Elut™. Isolute ® SLE+, Hydromatrix™