Sterile Medium 199 was developed for nutritional studies on cultured chick embryo muscle fibroblasts. Its unique components include purines, pyrimidines, and fat soluble components. Medium 199 is mainly used for virological studies and vaccine production. The highly complex medium can also be used without serum additions. However, the addition of FBS is recommended for long-term cultures. When working without FBS, growth factors, hormones and lipids, which are essential for cell growth, must also be added to the medium. ROTI ® Cell Medium 199 / Earle’s CELLPURE ® ready-to-use, sterile, with glutamine, w/o pyruvate Medium 199 contains L-glutamine and Earle's salts, but no pyruvate.