Adapted for use across diverse chemical handling environments, the Poly(A)-ClickSeq Library Prep Kit offers a streamlined approach for next-generation sequencing (NGS) applications focusing on polyadenylated RNA. This method, known as PAC-Seq, targets the 3' ends of eukaryotic mRNAs, providing cost-effective and efficient solutions for differential gene expression analysis and poly(A) site mapping. The kit employs 3'-azido-nucleotides (AzVTPs) during reverse transcription, followed by click-ligation to alkyne-functionalized adapters and PCR amplification to produce sequencing-ready libraries. Notably, this process eliminates the need for RNA purification, selection, or fragmentation, simplifying the workflow while maintaining high efficiency. Storing the kit at -20°C ensures its longev

• Category: NGS Kits
• Storage temp.: -20 °C