Prepared for use in regulated laboratory conditions, the X-Clickseq Library Prep Kit is designed for next-generation sequencing (NGS) applications. This innovative solution employs click-chemistry rather than enzymes to attach sequencing adapters to cDNA during library preparation. By incorporating 3'-azido-nucleotides during reverse transcription, the cDNA strands are blocked at random points, facilitating subsequent click-ligation to alkyne-modified adapters. Following PCR amplification, the resulting libraries are ready for sequencing analysis. Notably, this method offers advantages such as reduced enzymatic ligation requirements, a streamlined workflow, and minimal chimera formation, making it an efficient choice for both RNA and DNA input samples. The kit includes essential components like x-Primer Mix, Click Mix, Click Accelerant, Click Catalyst, i7

• Category: NGS Kits
• Storage temp.: -20 °C