This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research. High epitope affinity Highly specific detection of the target protein Unconjugated Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence anti-Desmocollin 3 mouse monoclonal, Dsc3-U114 50 μg/ml, monoclonal, affinity purified The mouse monoclonal antibody localizes Dsc3 present in all living epidermal layers, in glandular duct cells, in basal matrix cells and outer root sheath of hair follicles, in basal and suprabasal cell layers of stratified epithelia (e.g. vagina, tongue, esophagus), in basal layer of bladder urothelium and complex epithelium of trachea, in thymic reticulum cellsAll simple epithelia are negative. Intercalated disks of myocardium are negative. Polypeptide reacting: 109 kDa and 100 kDa polypeptides (desmocollin 3 splice isoforms at pI 5.2) from human epidermal desmosomes. Reactivity on cultured cell lines: most cultured cell lines derived from stratified squamous epithelia or squamous cell carcinomas are positive (e.g. A-431, HaCaT). Application Immunohistochemistry (IHC) - frozen: 1:10-1:100 (0.5-5 μg/ml, for better resolution preincubation (directly after fixation) with 0.05-0.2% Triton X-100, for 5-10 min, depending on tissue type, is recommended) Immunohistochemistry (IHC) - paraffin: 1:10-1:100 (0.5-5 μg/ml, microwave treatment recommended) Western Blot (WB): assay dependent IHC of mouse tongue with anti-Desmocollin 3 (1:10, courtesy of J.Heß, University Hospital Heidelberg) IHC of human oral mucosa with anti-Desmocollin 3 (1:10, courtesy of J. Heß, University Hospital Heidelberg) IHC of rat tongue with anti-Desmocollin 3 (1:10, courtesy of J. Heß, University Hospital Heidelberg) Not a medical device / Not an IVD product