This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research. High epitope affinity Highly specific detection of the target protein Unconjugated Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence anti-Cardiac Actin mouse monoclonal, AC1-20.4.2 50 μg/ml, monoclonal, affinity purified Ac1 represents an excellent marker for cardiac tissue; it discriminates fetal (cardiac) a-actin from all other actin isoforms. Fetal actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord. Mab Ac1-20.4.2 shows no cross reaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied. Polypeptide reacting: specific for fetal (cardiac) isoform of actin. Application Immunohistochemistry (IHC) - frozen: 1:10 (5 μg/ml; include 0.5 M NaCl in all washing buffers to enhance specificity) Immunohistochemistry (IHC) - paraffin: 1:10 (5 μg/ml; microwave treatment recommended) Western Blot (WB): 1:1,000 (50 ng/ml; include 1 M NaCl in all washing buffers to enhance specificity) Western blot analysis of human heart whole tissue lysate and with anti-Cardiac Actin antibody. Western blot analysis was performed on 20 μg human heart lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Cardiac Actin mouse monoclonal, AC1-20.4.2 (Cat. No. 690075) was diluted in blocking buffer (antibody concentration 0.05 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate. anti-Cardiac Actin mouse monoclonal, AC1-20.4.2, liquid, purified Not a medical device / Not an IVD product