This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research. High epitope affinity Highly specific detection of the target protein Unconjugated Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence anti-Keratin K20 mouse monoclonal, IT-Ks20.8 50 μg/ml, monoclonal, affinity purified IT-Ks20.8 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide recognized: protein IT (keratin K20; Mr 46,000; formerly also designated cytokeratin 20). Tested cultured cell lines: RT-4 Application Immunohistochemistry (IHC) - frozen: 1:10-1:100 (treatment with 0.02 % Triton X-100 recommended) Immunohistochemistry (IHC) - paraffin: 1:10-1:100 (protease treatment and/or microwave treatment recommended) Western Blot (WB): 1:1,000-1:5,000 Mouse colon (courtesy of J.Heß, University Hospital Heidelberg) Mouse colon (courtesy of J.Heß, University Hospital Heidelberg) Mouse colon (courtesy of J.Heß, University Hospital Heidelberg) Not a medical device / Not an IVD product