This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research. High epitope affinity Highly specific detection of the target protein Unconjugated Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence anti-Perilipin 1 (N-terminus) mouse monoclonal, PERI 112.17 100 μg/ml, monoclonal, affinity purified Perilipins build a family of phosphoproteins. The predominant forms in adipocytes, PLIN1 A and B arise by alternative RNA splicing from a single gene, generating polypeptides of 57 kDa and 46 kDa, respectively. The N-terminus, however, remains unchanged. The antibody reacts specifically with all PLIN1 variants located at the surface of intracellular storage lipid droplets present e.g. in the adrenal gland, sebaceous gland, adipocytes of white and brown adipose tissue and cultured cells such as 3T3-L1 adipocytes and cultured steroidogenic adrenal cortical and Leydig cells. It is also a useful pathological marker for steatogenesis e.g. in liver. It does not cross-react with adipophilin (ADRP, also named PLIN2) or TIP47 (also named PLIN3) proteins (or additional members of the PLIN/PAT-family, e.g. MLDP or OXPAT/PAT-1, also named PLIN5 or LSDP5). Tested reactivity on cultured cell lines: several human carcinoma cell lines; 3T3-L1 adipocytes. Application Immunocytochemistry (ICC): assay dependent Immunohistochemistry (IHC) - frozen: 1:200 (0.5 μg/ml) Immunohistochemistry (IHC) - paraffin: 1:200 (0.5 μg/ml, microwave treatment recommended) IHC analysis of human adipocytes in white adipose tissue of tumor microenvironment using anti-Perilipin 1 (N-terminus) antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 1 (N-terminus), PERI 112.17 (Cat. No. 690156) was diluted in PBS (antibody concentration 500 ng/ml) and incubated at 4 °C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 10x picture was acquired using microscopy (courtesy of J. Hess, University Hospital Heidelberg). IHC analysis of human cutaneous skin using anti-Perilipin 1 (N-terminus) antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 1 (N-terminus), PERI 112.17 (Cat. No. 690156) was diluted in PBS (antibody concentration 500 ng/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 10x picture was acquired using microscopy (courtesy of J. Hess, University Hospital Heidelberg). Not a medical device / Not an IVD product