This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research. High epitope affinity Highly specific detection of the target protein Unconjugated Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence anti-beta-actin, clone AC-15, mouse monoclonal 100 μg/ml, monoclonal, affinity purified Beta-Actin (42 kDa) is a highly conserved protein, which is able to polymerize to produce filaments. It is involved in cell motility, structure and integrity. Beta-Actin is a commonly used as a loading control for Western botting or as a housekeeping gene standard in qPCR. Application Immunohistochemistry (IHC) - paraffin: 1:50-1:500 (0.2-2 μg/ml; microwave treatment recommended) Western Blot (WB): 1:5,000 (20 ng/ml) IHC analysis of human colon using anti-beta-actin antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-beta-actin mouse monoclonal, clone AC-15 (Cat. No. 690974) was diluted in PBS (antibody concentration 500 ng/ml) and incubated at 4°C over-night. The secondary antibody HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J. Hess, University Hospital Heidelberg). IHC analysis of human head and neck squamous cell carcinoma using anti-beta-actin antibody (Cat. No. 690974). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-beta-actin mouse monoclonal, clone AC-15 was diluted in PBS (antibody concentration 500 ng/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J. Hess, University Hospital Heidelberg). Western blot analysis of HeLa lysate with anti-beta-actin antibody. Western blot analysis was performed on 20 μg HeLa lysate. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-beta-actin antibody mouse monoclonal, clone AC-15 (Cat. No. 690974) was diluted in blocking buffer (antibody concentration 0.02 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.4 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate. Not a medical device / Not an IVD product