This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research. High epitope affinity Highly specific detection of the target protein Unconjugated Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ASM-1 100 μg/ml, monoclonal, affinity purified 1A4/ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. Polypeptide reacting: specific for alpha-smooth-muscle isoform of actin (43 kDa). Tumors specificallly detected: leiomyosarcoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast. Tested cultured cell lines: stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts. Application Immunocytochemistry (ICC): Assay dependent Immunohistochemistry (IHC) - frozen: 1:200-1:1,000 (100-500 ng/ml) Immunohistochemistry (IHC) - paraffin: 1:200-1:1,000 (100-500 ng/ml, protease treatment and/or microwave treatment recommended) Western Blot (WB): 1:1,000 (100 ng/ml) Western blot analysis of human HeLa cell lysate with anti-alpha-Smooth Muscle Actin antibody. Western blot analysis was performed on either 10 μg, 5 μg or 2.5 μg of HeLa lysate. Cells were lysed in PBS by homogenization. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ASM-1 (Cat. No. 690001) was diluted in blocking buffer (antibody concentration 0.1 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate. IHC analysis of head and neck squamous cell carcinoma using anti-alpha-Smooth Muscle Actin antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ASM-1 (Cat. No. 690001) was diluted in PBS (antibody concentration 200 ng/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 10x picture was acquired using microscopy (courtesy of J.Hess, University Hospital Heidelberg). IHC analysis of human colon using anti-alpha-Smooth Muscle Actin antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ASM-1 (Cat. No. 690001) was diluted in PBS (antibody concentration 400 ng/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 10x picture was acquired using microscopy (courtesy of J.Hess, University Hospital Heidelberg). Not a medical device / Not an IVD product