This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research. High epitope affinity Highly specific detection of the target protein Unconjugated Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence anti-Desmocollin 1 mouse monoclonal, Dsc1-U100 50 μg/ml, monoclonal, affinity purified Mouse monoclonal antibody localizes Dsc1 present in suprabasal layers of interfollicular epidermis, in specific cell layers (e.g. in hair follicle root sheath) and in hassall bodies (spheroidal formations of densely packed reticulum-derived cells in the thymus). Reacts with 111 kDa (form 'a') and 103 kDa (form 'b') polypeptides (desmocollin 1 splice forms, both at pI 5.2) from human epidermal desmosomes. Reactivity on cultured cell lines: positive on primary keratinocytes, however, restricted to local piles of differentiated cells. All simple epithelia are negative. Host: mouse Antibody type: monoclonal Isotype: IgG1 Clone: Dsc1-U100 Immunogen: synthetic peptide corresponding to sequence present within the intracellular part of human desmocollin 1 (HQTLESVKGVGQGDTGRYATDWQC) UniprotID: Q08554 (Human) Synonym: desmocollin-1, DSC1, cadherin family member 1, desmosomal glycoprotein 2/3, DG2/DG3, CDHF1 Conjugate: unconjugated Purification: affinity chromatography Intended use: research use only Application: IHC, WB Reactivity: human, mouse, rat Application Immunohistochemistry (IHC) - frozen. 1:50-1:100 (0.5-1 μg/ml, preincubation (directly after fixation) with 0.05-0.2% Triton X-100, for 5-10 min, depending on tissue type, is recommended; without detergent pretreatment a cytoplasmic component might cross-react) Immunohistochemistry (IHC) - paraffin. 1:50-1:100 (0.5-1 μg/ml, microwave treatment recommended; a slight crossreactivity with a lymphocyte subpopulation is abolished by mild trypsinization after the microwave step) Western Blot (WB): Assay dependent IHC of rat skin with anti-desmocollin 1 (1:50, courtesy of J.Heß, University Hospital Heidelberg) anti-Desmocollin 1 mouse monoclonal, Dsc1-U100, liquid, purified IHC of mouse skin with anti-desmocollin 1 (1:50, courtesy of J.Heß, University Hospital Heidelberg) Not a medical device / Not an IVD product