Perfect separation behaviour in the gel. Protein ladders from Carl ROTH are acylated and pre-reduced, for optimal running behaviour in the gel. For each batch, intensity of bands is optimised and electrophoretically tested. All protein ladders are offered as ready-to-use solutions which may be applied directly to the gel. Please note: Reliable size determination of proteins cannot be performed using prestained markers. For this, we recommend the use of an unstained marker. ROTI ® Mark TRICOLOR XTRA ready-to-use, prestained ROTI ® Mark TRICOLOR XTRA is a tricolour-prestained ladder covering an extra range of molecular weights from 10 to 310 kDa in Tris-Glycine buffer. In order to visualise separation during the gel run and for better orientation in the gel, there is, besides the blue coloured proteins, one protein prestained in green (25 kDa) and two proteins in red (75 and 310 kDa). All bands excel by having a very even running behaviour and a good differentiation. ROTI ® Mark TRICOLOR XTRA is an ideal size ladder for controlling the gel run during SDS-PAGE and for reliable evaluation of transfer efficiency during Western Blot. Fig.: 15 % SDS-PAGE (Tris-Glycine)