Perfect separation behaviour in the gel. Protein ladders from Carl ROTH are acylated and pre-reduced, for optimal running behaviour in the gel. For each batch, intensity of bands is optimised and electrophoretically tested. All protein ladders are offered as ready-to-use solutions which may be applied directly to the gel. Please note: Reliable size determination of proteins cannot be performed using prestained markers. For this, we recommend the use of an unstained marker. ROTI ® Mark ALL BLUE ready-to-use, prestained ROTI ® Mark ALL BLUE is a ladder with 12 blue prestained proteins covering a range from 10 to 240 kDa in Tris-Glycine buffer. For better orientation in the gel the 25 kDa and the 75 kDa fragment are enhanced in their mass. ROTI ® Mark ALL BLUE is an ideal size ladder for controlling the gel run during SDS-PAGE and for reliable evaluation of transfer efficiency during Western Blot. Fig.: 15 % SDS-PAGE (Tris-Glycine)