Tested for use in tissue culture.At biological pH, HEPES is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 20 = 7.55). Thus, this Good's buffer is used for a wide range of applications in biological research: • In tissue culture to buffer cell culture media in air • As a component in cell culture media like DMEM, RPMI, Hanks’ solution • In biological solutions for the adjustment and maintenance of pH • For homogenization of tissue and in the preparation of cytosolic and nuclear cell extracts • As a buffer component in gel filtration and affinity chromatography Tested for use in tissue culture.At biological pH, HEPES is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 20 = 7.55). Thus, this Good's buffer is used for a wide range of applications in biological research: • In tissue culture to buffer cell culture media in air • As a component in cell culture media like DMEM, RPMI, Hanks’ solution • In biological solutions for the adjustment and maintenance of pH • For homogenization of tissue and in the preparation of cytosolic and nuclear cell extracts • As a buffer component in gel filtration and affinity chromatography